Fig 1: Lipid droplet associated proteins and lipid uptake receptor expression differ between mitotane-sensitive and -resistant cell lines. Western blotting data represents protein expression of (A) PLIN1, PLIN2, PLIN3, PLIN4, and B actin in H295R, MUC1, and HEPG2. (B) Graphical representation of relative mRNA expression of Plin2 and Plin3 in H295R and MUC-1 cell lines. (C) Graphical representation of lipid uptake receptor expression CD36, LDLR, SCARB1, and LRP1 in H295R and MUC-1. (D) Western blot data represents protein expression of HSL in H295R, MUC-1, and HEPG2. (E) Graphical representation of relative mRNA expression of Lipe H295R and MUC-1 cell lines Western blot data represents protein expression of (G) SOAT1 and (H) DGAT1 HSL in H295R, MUC-1, and HEPG2. For western blot analysis, ß actin was used as a normalizing protein. Images are representative of 3 independent blots, numerical annotation is representative of semiquantitative analysis using ImageJ relative to H295R. mRNA expression is represented as fold change relative to H295R, B2M was used as a normalizing gene. Data are represented as mean ± SEM (n = 3), statistical comparisons were performed using a 2-tailed t-test or a 2-way ANOVA followed by Tukey post hoc analysis. Statistical significance is denoted as actual P values relative to vehicle control or as otherwise indicated.